Mechanistic Insight Into Responses of Translational Mishaps

Co-translational  quality  control  is  triggered  as a response  to  translational  stalling  events.  Yet,  different molecular mechanisms are employed for the recognition of these  stalls  and  to  trigger  downstream  rescue  and  quality control pathways. While the recognition of individual stalled ribosomes is poorly understood, the use of collided ribosomes as a proxy for the recognition of translation problems in the cell is conserved from bacteria to humans. In eukaryotes, co-translational quality-control processes triggered by ribosome collisions  accomplish  several  tasks  and  eventually  trigger stress response signaling pathways. We employ cryo-EM to provide mechanistic understanding of these processes.